The Four Stages of Wound Healing


Use of ozone for wound healing is a two-phase process:

Phase 1 – Kill the bacteria and viruses in the wound – use ozone gas – 60 to 80 gamma, full ozonated water or strong ozonated oils – Three times per day for first two days

Phase 2 – Promote healing through increased oxygenation – ozone gas – 20 gamma or milder ozone oil – Three times per day for two weeks

See Bocci on Ozonated Oils study


There are also four stages of wound healing:

1). Hemostasis:

Damaged blood vessels constrict, platelet plugs form and blood coagulates. Platelets and damaged cells initiate reactions ultimately resulting in fibrin threads and clot formation.

2). Inflammation Phase:

Swelling and warmth often associated with pain.
The inflammatory response causes the blood vessels to become leaky releasing plasma and neutrophils into the surrounding tissue.This cleans up debris and fights infection.
The area exhibits redness and swelling accompanied by heat and pain.
Macrophages are able to phagocytize bacteria and provide a second line of defense. They also direct the next phase of healing.

3). Proliferative Phase (Proliferation, Granulation and Contraction):

The granulation stage starts four days after wounding and can last up to three weeks in acute wounds.
Fibroblasts secrete the collagen framework upon which further dermal regeneration occurs.
Pericytes regenerate the outer layers of capillaries and the endothelial cells which produce the lining.
Specialized fibroblasts are responsible for wound contraction. Keratinocytes are responsible for epithelialization.

4). Remodeling or Maturation Phase:

The healing process involves remodeling the dermal tissues to produce greater tensile strength by the fibroblasts.
Remodeling can take up to two years after wounding.

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