Additional Studies 2018-05-16T12:19:31+00:00

Miscellaneous Studies for Ozone

  • Diabetic Foot
  • Platelet Aggregation
  • Blood Brain Issues, Stroke, Parkinsons
  • Pain
  • Leaky Gut
  • Cerebral Palsy
  • Tarsal Tunnel
  • Urology
  • Emphysema

The Bocci Collection

Prof. Velio Bocci

  1. Induction of interferon gamma on human leucocytes.
  2. Induction of tumor necrosis factor (TNF-alpha) on human leucocytes.
  3. An attempt to define conditions for optimal induction of cytokines
  4. Cytokine production and glutathione levels in human erythrocytes.
  5. Evaluation of immunological parameters
  6. Production of transforming growth factor 1 by human blood after ozone treatment.
  7. Generation of (ROS) after exposure of human blood to ozone.
  8. Effects on the total antioxidant status and on interleukin-8 production
  9. Effects of ozone on human platelets.
  10. Release of factors from ozonated human platelets.

View Document [PDF]

Guidelines and Treatment Strategies

Renate Viebahn-Hänsler, Olga Sonia León Fernández & Ziad Fahmy

When used in specific diseases and conditions, medical ozone produces the same or similar therapy results worldwide. Improper application in the form of erratic methods and doses is the most frequent cause of ineffectiveness and adverse effects—and is always the cause of violent controversies. For this reason, the medical societies for ozone application have set up treatment protocols as basis for standards and guidelines, revised and published as a result of the most recent research and 30 years of experience (Beck et al. 1998; Knoch et al. 2009). They have been used in the standardization of applications, indications, concentrations, doses, and frequency of treatment as based on the mechanism of action and the pharmacology of ozone.
View Document [PDF]

Ozone Therapy and Its Scientific Foundations ISCO3

Dr. Adriana Schwartz and Dr. Gregorio Matínez Sánchez, Madrid, 2012

In recent years, ozone therapy as an effective therapeutic method has become more developed and well known. Russia and Cuba have recognized it in their legislation; it is regulated in more than 76% of the Autonomous Regions of Spain; and in Italy four Regions have specified the criteria for practicing it, in addition to two favorable court decisions. Ozone therapy is characterized by the simplicity of its application, its great effectiveness, good tolerance, and by the virtual absence of side effects. This document, based on the latest books and scientific articles on the subject, updates the recent findings that justify, from the scientific point of view, the medical use of ozone. For many years the application of ozone in medical practice was not well accepted due to unfounded ideas about its toxicity in relation to the high concentrations used in industry. As with any healing technique, ozone therapy is dependent on the dosage.

View Document [PDF]

Ozone Therapy in Russian Practice

Nizhny Novgorod, Russia, 2008

The Health Manual offers a variety of information on recognized methods and techniques for clinical application of ozone therapy in various pathologies. It briefs the readers on ozone properties and therapeutic effect of ozone-oxygen mixtures administered in different diseases, including the dosage and schemes of treatment that are used by Russian doctors.

Ozone therapy in Practice is a practical guide for doctors of various specialities, who use medical ozone in their practice, as well as for interns, residents in medicine and senior medical students.

View Document [PDF]

Madrid Declaration On Ozone Therapy

Madrid, 2010

Approved at the “International Meeting of Ozone Therapy Schools” held at the Royal Academy of Medicine in Madrid on the 3rd and 4th of June, 2010, under the auspices of the Spanish Association of Medical Professionals in Ozone Therapy (AEPROMO)

View Document [PDF]

Therapeutic efficacy of ozone in patients with diabetic foot: Abstract

Oxidative stress is suggested to have an important role in the development of complications in diabetes. Because ozone therapy can activate the antioxidant system, influencing the level of glycemia and some markers of endothelial cell damage, the aim of this study was to investigate the therapeutic efficacy of ozone in the treatment of patients with type 2 diabetes and diabetic feet and to compare ozone with antibiotic therapy. A randomized controlled clinical trial was performed with 101 patients divided into two groups: one (n = 52) treated with ozone (local and rectal insufflation of the gas) and the other (n = 49) treated with topical and systemic antibiotics. The efficacy of the treatments was evaluated by comparing the glycemic index, the area and perimeter of the lesions and biochemical markers of oxidative stress and endothelial damage in both groups after 20 days of treatment. Ozone treatment improved glycemic control, prevented oxidative stress, normalized levels of organic peroxides, and activated superoxide dismutase. The pharmacodynamic effect of ozone in the treatment of patients with neuroinfectious diabetic foot can be ascribed to the possibility of it being a superoxide scavenger. Superoxide is considered a link between the four metabolic routes associated with diabetes pathology and its complications. Furthermore, the healing of the lesions improved, resulting in fewer amputations than in control group. There were no side effects. These results show that medical ozone treatment could be an alternative therapy in the treatment of diabetes and its complications.

Martínez-Sánchez G, Al-Dalain SM, Menéndez S, Re L, Giuliani A, Candelario-Jalil E, Alvarez H, Fernández-Montequín JI, León OS.

Source – Center of Studies for Research and Biological Evaluation (CEIEB-IFAL), University of Havana, Havana 10400, Cuba.

Platelet inhibition in patients with vascular diseases submitted to ozone therapy treatment.

The aim of this study is to verified whether there is or not inhibition of platelet aggregation after ozone treatment, in patients diagnosed with an ischemic or neuroinfectious diabetic foot, chronic venous insufficiency and subacute arterial ischaemia. Ozone was applied daily, by rectal insufflation, at a concentration of 50 mg/L and 200 mL, in a cycle of 20 sessions. Platelet aggregation was measured at the beginning and at the end of ozone treatment. Platelet aggregation in platelet rich plasma (200 µL) was induced by adding increasing concentrations of adenosine diphosphate, collagen and ephinephrine. We observed that at the end of the treatment, platelet aggregation percentage was reduced to a mean of 14 to 28%, in comparison with the initial values.

Díaz A., García M., Piña and Menéndez S. (Cuba); Nelaeva I.A. (Russia) Click here for more

Ozone therapy in treatment of discirculatory encephalopathy. Psychological aspects.

The cerebrovascular pathology and its dangerous sequela – the cerebral insult is the most highly social and medical problem not only in Russia but all over the world. This disease takes the second place (21.4%) among the general mortality rate in Russia. The discirculatory encephalopathy is the slowly progressing insufficiency of the brain circulation. The nootropil, vascular drugs, antiaggregates, vitamins, antioxidants and antidepressants are used in the treatment of discirculatory encephalopathy traditionally. The main index for the use of ozone therapy treatment in patients with discirculatory encephalopathy is the insufficient oxygen supply of the brain and its assimilation. All the patients were undergone the clinical neurologic examination in addition to ophthalmoscopy, craniography and experimental psychological check up. The 24 patients with the discirculatory encephalopathy (I and II stages) have been examined and treated. The intravenous drop infusions of the ozone physiological solution were carried out daily. The cycle of ozone therapy treatment was 8 – 10 procedures. The analysis of the clinical evolution of the experimental psychological investigation, at the end of the ozone treatment, showed the decrease of the main complaints in 17 patients with discirculatory encephalopathy (70.8%). So, our previous data demonstrated the good effects achieved using ozone therapy in the treatment of discirculatory encephalopathy.

Nelaeva I.A. (Russia)

Use of ozone therapy in the stroke.

The cerebrovascular disease is one of the main problems in medical practice, because of the high tendency to produce sequels in those who survive, limiting their physical capacities. The purpose of this study was to evaluate the effectiveness of ozone therapy in the ischemic cerebrovascular disease, in acute phase. One hundred patients, with this diagnosis, that attended the Neurological Institute during a period of one year, were evaluated. Ozone by rectal insufflation was daily administered (at an ozone concentration of 40 mg/L and 200 mL), for a total of 15 sessions. These patients were evaluated by clinical examination, blood test and their validity through the multidimensional evaluation method, at the beginning and at the end of the treatment. Antiaggregant medication was associated. The clinical stage improved in 80% of patients treated with ozone therapy, with a satisfactory increase of their quality of life.

Castillo P. and Salas T. (Cuba); AEPROMO, 2012 Revista Española de Ozonoterapia Vol.2 No. 2. Supplement 1, 2012, ISSN 2174-3215 III International Congress of AEPROMO “The ozonetherapy in the medical agenda” Spanish Association of Medical Professionals in Ozone Therapy.

The Contributions of Ozone Therapy to Parkinson’s Disease.

Parkinson’s Disease is a condition attributed to a degenerative process in the dopominergic pathways, characterized by trembling, muscular rigidity, akinesia, alterations of posture and balance, steppage gait, lack of facial expression and excessive salivation.Although the cause of nigral degeneration and degeneration of the neostriatum is unknown, there exist theories which point to oxidative stress as the initial trigger.There exist a multitude of scales to evaluate a patient who suffers from Parkinson’s disease: the Hoehn-Yahr scale, the Columbia University Rating Scale (CURS), the Activities of Daily Living scale (ADL), etc.

Currently, pharmaceutical medicines allow the sufferers of Parkinson’s Disease to efficiently relieve the majority of their symptoms which, combined with various physiotherapeutic techniques and Ozone Therapy, let the patients maintain an acceptable quality of life.The objective of this study was to evaluate the effects of

Ozone Therapy on the appearance and subsequent evolution of the disease. The present study began in April, 2011 and included 30 patients. The patients were classified in stages based upon the Hoehn-Yahr scale. 15 of these patients received Ozone Therapy by rectal insufflations or by autohemotransfusion, as well as maintaining their normal Parkinson’s medications. The other 15 patients constituted the Control Group.

The study was structured to evaluate the results after one year of treatment; however a check was made after six months. The patients were evaluated using the CURS scale and the condition of specific activities of daily living (ADL). Preliminary results showed a drop in the items of the CURS scale in 60% of the patients and an improvement in the ADL in 50% in comparison to the Control Group. Since these are preliminary results, it would be premature to draw conclusions; however so far, everything leads us to believe that Ozone Therapy will have benefits.

Francisco José Martín Florido, Doctor in Medicine and Surgery. Expertise in anti-aging medicine from the University of Seville.Expertise in Ozone Therapy from the University of Seville. Director of the Clinic Medicentro in Huelva, Spain. Coordinator of Ozone Therapy courses at the University of Seville. E-mail:

Pain. Treatment of Neuropathic Pain by Ozone Injections.

With the purpose of knowing about ozone effectiveness in neuropathic pain, we studied the use of ozone injections in neuropathic pain patient attending our Pain Clinic. The pain syndromes treated were the following:

Carpal tunnel syndrome, cubital tunnel syndrome at the elbow, posterior tarsal tunnel syndrome, anterior tarsal tunnel syndrome, traumatic brachial plexus injuries, Pancoast syndrome, complex regional pain syndrome, narrow cervical canal, narrow lumbar canal, diabetic neuropathy, acute herpes zoster pain, post herpetic neuritis, trigeminal neuralgia, esphenopalatin neuralgia and occipital neuralgia.

Ozone 10 μg / mL was injected directly at the pathologic process site, such as carpal tunnel or cubital tunnel, 3 to 5 mL volume. In the case of brachial plexus pathology either posttraumatic or compressive (Pancoast syndrome) ozone was injected directly in the brachial plexus by the inter scalene approach, 20 mL volume. In the painful pathologies caused by nerve root compression at the cervical level by herniated disc or narrow cervical spinal canal, ozone was applied at the epidural interlaminar thoracic level, T1-T2 or T3-T4, 20 mL volume or directly on the brachial plexus by the inter scalene approach, 20 mL volume. In the cases of lumbar root pain caused by herniated disc or narrow lumbar spinal canal, we used the epidural caudal approach, 20 a 40 mL volume or the lumbar para vertebral approach, 20 mL directly over the nerve root or a combination of both techniques. In the cases of herpes zoster pain or diabetic mono neuropathy, ozone was applied according to the neuropathy location. Example at the thoracic level we injected the ozone by the thoracic para vertebral approach directly over the affected nerve root, 10 mL volume or at the intercostal space, 10 mL volume. When the neuropathy affected cervical roots, we injected the ozone directly over the brachial plexus by inter scalene approach, 20 ml volume. In the case of trigeminal neuralgia, ozone was injected directly over the affected branch 5 mL volume and in the case of sphenopalatine neuralgia ozone was injected in the sphenopalatine fossa, 5 mL volume. Patients benefited from ozone application between 75% and 90% of cases, varying according to the pathology treated. We achieved pain relief over 70% according to visual analog scale for more than six months. There were no serious complications.

Dr. Jaime Rebeil Félix, Head of Pain Clinic of General Hospital in Hermosillo, Sonora, Mexico. Revista Española de Ozonoterapia Vol.2 Supplement 1 2012, ISSN 2174-3215; II International Medical Ozone Federation Congress. IMEOF; III Mexican Ozonetherapy Association Congress. AMOZON; “For the Integration of Ozonetherapy into the Conventional Medicine.”

Joint injections with Ozone: Regenerating Joints and Eliminating Pain.

Joint injections with Ozone is a regenerative and pain relieving procedure that involves the proliferative principles of prolotherapy, the cell membrane repolarizing principles of neural therapy, the stem cell stimulation of homeopathic therapy, and the metabolic principles of ozone therapy. The area being treated is first injected with a 10% solution of glucose combined with procaine, B-vitamins, anti-inflammatories, homeopathics, and minerals. A second injection of ozone is then applied. Because ozone is a gas, it extensively distributes throughout tissues. This allows for the treatment of a very wide area with a minimal number of injections. Joint injections with Ozone is applicable and usually effective in any clinical situation in which there is pain and/or ligament, cartilage, or bone damage.

Frank A. Shallenberger, MD, HMD, ABAAM; President, American Academy of Ozonotherapy

Treatment of Increased Intestinal Wall Permeability (Leaky Gut) and the Associated Immune System Dysfunctions with Colonic Hydrotherapy Followed by Rectal Insufflations and Minor Autohemotherapy with Oxygen-Ozone.

Autoimmune diseases when considered as a group are the third cause of sickness and death worldwide nowadays. Among the causes of autoimmunity, genetic predisposition, immune regulation abnormalities and environmental factors are considered the key elements for developing such diseases. One of the most relevant environmental factors is the disruption of the intestinal wall, a process known as increased intestinal wall permeability or leaky gut, where the contents of the intestinal lumen penetrate the gut barrier, breaching through tight junctions and desmosomes that glue intestinal wall cells together, and exposing proteins of dietary origin to the immune system within the intestinal wall.

This process is manifested first as food allergies or intolerance, but as it goes on, the increased permeability in the gut allows diet proteins to get into the bloodstream and generate antibodies against our own cells. More than 40 autoimmune diseases have been traced back to predictive auto-antibodies that can be present for more than 7 years before the onset of autoimmunity symptoms and the diagnosis of a specific condition, being the most prevalent ones arthritis, thyroiditis, type I diabetes and celiac disease.

Colonic hydrotherapy –the administration of temperature and pressure controlled water into the colon-, is a very effective therapy aimed at the mechanical removal of immune complexes with dietary proteins nested in the intestinal wall, but the abnormalities in the regulation of the local and systemic immune system are harder to treat. Oxygen-ozone gas mixture administered by rectal insufflation after the colonic hydrotherapy provides the means to effectively re-establish a healthy immune response in the gut, since ozone has a well-known dose-dependent suppressing or stimulating effect upon the immune system. Minor autohemotherapy with oxygen-ozone at a high dose (40 μg) has been effectively used as a means to treat allergies and other immune system abnormalities.

In this paper we review a series of 20 cases of leaky gut treated in our clinic with a series of 3 to 5 colonic hydrotherapies followed by 20 to 30 rectal insufflations of oxygen-ozone at a concentration ranging from 10 to 35 μg and a volume ranging from 100 to 180 mL. All the patients also received weekly minor autohemotherapies (4 to 6) with 20 to 40 μg of ozone. 6 of our patients suffered from chronic colitis with abdominal distention, spastic pain and constipation; 3 of them were diagnosed with irritable bowel syndrome; 4 suffered from food intolerances (lactose, seafood, wheat); 5 of these patients showed signs of subclinical hypothyroidism confirmed by lab, and the other two had psoriasis. All of the patients had also electrical evidence – as established by bioimpedance, ESTECK Complex Scanner, LD Technologies, USA – of digestive dysfunction (food allergies or intolerance), thyroid gland electric imbalance and/or elevated function of the immune system.

After the treatment, 15 of the patients had cleared out the digestive dysfunction and the immune system abnormalities both clinically and electrically, 2 of them persisted with digestive dysfunction although they reduced the signs and symptoms of comorbidities and 3 of the patients persisted with both digestive dysfunction and comorbidities. The success rate of the treatment regime (75%) makes the administration of oxygen-ozone a very effective therapy to normalize the immune system abnormalities both systemically and in the gut, providing a innovative solution to the increasing problem of intestinal wall permeability and the associated autoimmune abnormalities.

Luis David Suárez Rodríguez MD Lc Ac. Centro de Medicina Integrativa SANAR, S.C., Playa del Carmen, Q. Roo, México.

Ozone Therapy for the Diabetic Foot: A case report

In many cases of diabetic foot, ozone therapy (OT) can be the one method which can save the limb from amputation. Since the great report of Rokitansky, given at the IOA World Congress, Washington DC, 1983, it is generally accepted that Ozone therapy can avoid roughly 50% of amputations. OT offers the chance to approach the problem externally / topically with local application of ozone via “bagging”, as well as internally / systemically with the major autohemotherapy with ozone and, in extreme cases, also with injections of the O2-O3 gas mixture directly into the femoral artery. The local application of ozone in high concentrations by “bagging” is meant to sterilize the wounds. In a second phase, lower ozone concentrations will help the healing process. In the beginning, it should be given daily or at least three times per week. The systemic OT via major auto-hemotherapy will increase the oxygen saturation in the erythrocytes, increase the liberation of this oxygen in the peripheral capillaries, modify rheological properties of the blood, avoid “sludging” of red blood cells, improve all metabolic steps of cell respiration, it will help control blood sugar levels, as well as upgrade the patient’s immune response and general condition. Intra-arterial injection of ozone must be reserved for extreme cases only. This is a more difficult technique, requiring more specialized know-how and somewhat more accident-prone than most other forms of OT, which is why it is no longer generally recommended by the German Medical Society for Ozone Therapy.

Heinz Konrad; Private Medical Office, São Paulo, Brazil Email:

Results of Conservative Treatment of Tarsal Tunnel Syndrome: Medical Ozone Infiltration and Surgery.

The tarsal tunnel syndrome (TTS) is an extrinsic or intrinsic neuropathy of the foot, secondary to compression of the tibial nerve (TN) or one of its terminal branches (medial plantar n., Lateral plantar n. Medial calcaneus n.) in the tarsal canal or channel Richet. The release thereof was first described by Keck and Lam in 1962. The TTS is similar to carpal tunnel syndrome, although much less frequent. In the current nomenclature we speak of posterior and anterior tibial nerve, naming them as deep peroneal and tibial nerve. We believe that the frequency of this disease which is greater than has hitherto been considered. We will try to clarify the pathogenesis, diagnosis and treatment of this condition, adding to the studies reviewed our experience.

Since 1999 we have treated in our clinic 588 TTS, we systematically performed a thorough examination, studies Rx laden feet, and in cases not cured with injections of medical ozone (MO), EMG, ENG, NMR, etc. All patients have been treated by the same medical team and those who have been cured of their symptoms with the infiltration of TTS (not associated pathologies) have been treated surgically. As we have made treatment regimen TTS infiltration fortnightly up to 3, with 2% mepivacaine without epinephrine, we have waited three minutes to inject 10 mL. Medical ozone at a concentration of 30 μg / mL.

Cured by MO 490 infiltration TTS, 10% with the first, 30% with the second and 40% in the third. The remaining 20% were treated surgically. We have intervened 98 TTS in 84 patients by 2% of our interventions in pathologies of the foot, in this period we have operated in our Unit 6 240 feet. The results have been excellent, 80% good in 18% and we just had two bad results.The TTS is a common foot, the results with OM infiltration lead to cure in 80% of cases. In the remaining patients either performed surgical treatment gives excellent results. Do not forget to treat pathologies that produce TTS.

Juan López-Laserna Ruiz, Chief of the Foot and Ankle Unit. San Juan de Dios Hospital. Cordoba.

Ozone Therapy in Urology.

The application of ozone in the most frequent pathologies of urology has had a high level of efficacy, such as in prostatic hyperplasia, prostate cancer, Balanitis, stricture of urethra, erectile dysfunction, ejaculation Peyronie disease, interstitial cystitis, human papillomavirus virus and mixed cervical-vaginitis. In this study we analyze the implementation of the ozone therapy depending on the site of affectation and using a combination of different techniques: Autohemoterapy Mayor, local, subcutaneous, directly in the injury and by instillation. In general it was observed a fast evolution of the symptoms and in many of the cases the total healing.

The objective was to provide a better quality of life for the patient from different point of view: curative, preventive or palliative in the treatment of the 10 most common pathologies in urology. More than 80% of patients showed improvement in their symptoms, as it is the case of prostatic hyperplasia in where a reduction in the size of the prostate were 10% to 30%. In addition, elimination of residual urine was observed after treatment. In the case of advanced prostate cancer was applying autohemoterapy in combination with ultraviolet light getting the Prostatic Antigen (PAS) reduction and improving the general health status of the patient.

In the topical application of ozone in Glans affected from balanitis a success has been observed because 90% of the patients improved their symptoms. The same results were observed in topical and intravaginal application in cases of cervico-vaginitis. In Peyronie’s disease within fibrosis application after several applications of ozone there was observed a reduction of more than 90% of the injury. The application of ozone in corpus cavernosum also increases vascular flow improving the rigidity of the erection.

The subcutaneous application of ozone in Raphe decreased the hypersensitivity of the Glans getting better ejaculatory control. Ozone applied directly into the male urethra with stenosis, improves urinary flow and urethral caliber as this technique is also used to eliminate the infection by Human Papilloma Virus. In cases of interstitial cystitis intravesical ozonized water was applied repeatedly and the improvement occurs in a short time with long periods of remitting without irritation and infection. All applications of ozone therapy in patients suffering from urological pathology results in an important improvement and in many cases healing. This work shows the great usefulness of ozone therapy as a great tool in the medical practice with surprising results that offer the patient a better quality of life.

Eduardo Martín García Villanueva; Medica Angelus. México D.F., C.P. 07300

Rectal ozone therapy for patients with pulmonary emphysema: Abstract

BACKGROUND: Ozone therapy may stimulate antioxidant systems and protect against free radicals. It has not been used formerly in patients with pulmonary emphysema.

AIM: To assess the effects of rectal ozone therapy in patients with pulmonary emphysema.

MATERIAL AND METHODS: Sixty-four patients with pulmonary emphysema, aged between 40 and 69 years, were randomly assigned to receive rectal ozone in 20 daily sessions, rectal medicinal oxygen or no treatment. Treatments were repeated three months later in the first two groups. At baseline and at the end of the study, spirometry and a clinical assessment were performed.

RESULTS: Fifty patients completed the protocol, 20 receiving ozone therapy, 20 receiving rectal oxygen and 10 not receiving any therapy. At baseline, patients on ozone therapy had significantly lower values of forced expiratory volume in the first second (fEV1) and fEV1/forced vital capacity. At the end of the treatment period, these parameters were similar in the three treatment groups, therefore they only improved significantly in the group on ozone therapy. No differences were observed in other spirometric parameters.

CONCLUSIONS: Rectal ozone therapy may be useful in patients with pulmonary emphysema.

Calunga JL, Paz Y, Menéndez S, Martínez A, Hernández A.

Source – Centro de Investigaciones del Ozono, La Habana, Cuba.