Studies On Ozone Skin Use 2018-01-21T21:57:58+00:00

Skin and Ozone Studies

Ozonated Oils and their action

It is most interesting that ozone, an unstable gas, can be stably trapped as an ozonide between a double bond of a PUFA: dðCH2Þ7dO3dðCH2Þ7CH3. When the ozonated oil is layered over the ulcer’s exudate at the oil/water interface, the ozone moves slowly into the water and, by reacting with biomolecules, generates a steady flow of H2O2. The effects of sterilization and improved oxygenation are responsible for the accelerated cicatrization (become healed by the formation of scar tissue). In comparison to pharmaceutical creams often containing useless antibiotics and growth factors, once ozonated oil is known and used, it will be extremely beneficial to millions of patients.

From: Scientific and Medical Aspects of Ozone Therapy. State of the Art Velio Alvaro Bocci Department of Physiology, University of Siena, Siena, Italy Received for publication May 26, 2005; accepted August 26, 2005 (ARCMED-D-05-00204).

The dual action of ozone on the skin.

-Valacchi G, Fortino V, Bocci V.


Department of Physiology, University of Siena, Siena 53100, Italy.


The aim of this brief review is to summarize the recent literature on the effect of ozone (O3) on cutaneous tissues. Recently it has been reported that a chronic contact with O3 can be deleterious for the skin. Our group and others have shown a progressive depletion of antioxidant content in the stratum corneum and this can then lead to a cascade of effects resulting in an active cellular response in the deeper layers of the skin. Using an in vivo model we have shown an increase of proliferative, adaptive and proinflammatory cutaneous tissue responses. On the other hand the well known activity of O3 as a potent disinfectant and oxygen (O2) donor has been also studied for therapeutic use. Two approaches have been described. The first consists of a quasi-total body exposure in a thermostatically controlled cabin. This treatment has proved to be useful in patients with chronic limb ischaemia. The second approach is based on the topical application of ozonated olive oil in several kinds of skin infection (from soreness to diabetic ulcers, burns, traumatic and surgical wounds, abscesses and skin reactions after radiotherapy). We and other authors have observed a striking cleansing effect with improved oxygenation and enhanced healing of these conditions. It is now clear that, on the skin, O3, like other drugs, poisons and radiation, can display either a damaging effect from a long exposure or a beneficial effect after a brief exposure to O2 and O3 or to the application of ozonated oil to chronic wounds.

Wound Repair Regen. 2011 Jan-Feb;19(1):107-15. doi: 10.1111/j.1524-475X.2010.00649.x. Epub 2010 Dec 6.

Ozonated sesame oil enhances cutaneous wound healing in SKH1 mice.

-Valacchi G, Lim Y, Belmonte G, Miracco C, Zanardi I, Bocci V, Travagli V.


Dipartimento di Scienze Biomediche, Università degli Studi di Siena, Siena, Italy.


Ozone is well recognized as a bactericidal agent and its beneficial effect on wound healing could be a consequence of this property. Because ozone itself does not penetrate the cells but immediately reacts with polyunsaturated fatty acids, its effects should be the results of oxidative reaction. For this reason, ozonated oils could be a way to deliver ozone messengers to the skin. This paper evaluated the therapeutic effects of three different grades of ozonated sesame oil in acute cutaneous wounds made in the skin of SKH1 mice. Specifically, wound closure rate, histological parameters, and the level of key proteins such as vascular endothelial growth factors and cyclin D1 have been analyzed in relation to the peroxide level present in the ozonated oil. Treatment with moderately ozonated sesame oil–expressed as peroxide value about 1,500)–has a faster wound closure rate in the first 7 days than treatment with oil containing either lower or higher peroxide value, and even with controls. Moreover, under the same treatment, an earlier and higher response of cells involved in wound repair, a higher angiogenesis, as well as an enhanced vascular endothelial growth factors and cyclin D1 expression were observed. The present study shows the validity of ozonated sesame oil in cutaneous wound healing and emphasizes the importance of the ozonation grade.

© 2010 by the Wound Healing Society.

Ozone and ozonated oils in skin diseases: a review.

-Travagli V, Zanardi I, Valacchi G, Bocci V.


Dipartimento Farmaco Chimico Tecnologico, Università degli Studi di Siena, Viale Aldo Moro 2, 53100 Siena, Italy.


Although orthodox medicine has provided a variety of topical anti-infective agents, some of them have become scarcely effective owing to antibiotic- and chemotherapeutic-resistant pathogens. For more than a century, ozone has been known to be an excellent disinfectant that nevertheless had to be used with caution for its oxidizing properties. Only during the last decade it has been learned how to tame its great reactivity by precisely dosing its concentration and permanently incorporating the gas into triglycerides where gaseous ozone chemically reacts with unsaturated substrates leading to therapeutically active ozonated derivatives. Today the stability and efficacy of the ozonated oils have been already demonstrated, but owing to a plethora of commercial products, the present paper aims to analyze these derivatives suggesting the strategy to obtain products with the best characteristics.

Therapeutic Effects of Topical Application of Ozone on Acute Cutaneous Wound Healing


This study was undertaken to evaluate the therapeutic effects of topical ozonated olive oil on acute cutaneous wound healing in a guinea pig model and also to elucidate its therapeutic mechanism. After creating full-thickness skin wounds on the backs of guinea pigs by using a 6 mm punch biopsy, we examined the wound healing effect of topically applied ozonated olive oil (ozone group), as compared to the pure olive oil (oil group) and non-treatment (control group). The ozone group of guinea pig had a significantly smaller wound size and a residual wound area than the oil group, on days 5 (P<0.05) and 7 (P<0.01 and P<0.05) after wound surgery, respectively. Both hematoxylin-eosin staining and Masson-trichrome staining revealed an increased intensity of collagen fibers and a greater number of fibroblasts in the ozone group than that in the oil group on day 7. Immunohistochemical staining demonstrated upregulation of platelet derived growth factor (PDGF), transforming growth factor-β (TGF-β) and vascular endothelial growth factor (VEGF) expressions, but not fibroblast growth factor expression in the ozone group on day 7, as compared with the oil group. In conclusion, these results demonstrate that topical application of ozonated olive oil can accelerate acute cutaneous wound repair in a guinea pig in association with the increased expression of PDGF, TGF-β, and VEGF.