IV Ozone Therapy (Major Autohemotherapy - MaH)

Uses, Benefits, Side Effects, Protocols, and Ozone Therapy Training
Table of Contents

At a glance:

  • IV ozone therapy is the administration of medical grade ozone and oxygen gas into the blood.  A small amount of blood is pulled from the body, mixed with ozone gas, then reinfused.
  • Benefits of IV ozone therapy include immune modulation, improved oxygen efficiency, increased microcirculation, and modulating oxidative stress.  Ozone therapy is used for a wide variety of diseases but is most commonly applied for lyme, autoimmune disorders, mold toxicity, cancer, and general optimization.
  • People generally experience an increase in energy, cognition, and a decrease in pain.
  • The safety of ozone therapy has been demonstrated to have a lower adverse event rate than the use of aspirin.  The most common negative side effect is a herxheimer reaction because it activates the immune system which kills a lot of pathogens and can leave the patient feeling unwell for a short time.
  • Ozone therapy training for practitioners is available here
  • The protocol for autohemotherapy ozone involves drawing 60cc of blood, mixing it with ozone gas, and then infusing it back into the patient.

What is IV ozone therapy major autohemotherapy (MaH) ?

IV ozone therapy is the administration of pure medical ozone gas into the vein. 

During the treatment, a small amount of blood is withdrawn from the vein, put into a special ozone bag.  Then, ozone gas is infused into the blood and it is infused back into the vein over the course of 30 minutes.

It is often called major autohemotherapy or MaH.  

What are the benefits of IV ozone treatments?

Ozone therapy stimulates the body toward homeostasis by creating a mild, acute oxidative stress and activates the NRF2 pathway (the same pathway activated during exercise and fasting).  Among many mechanisms of action, ozone therapy is able to increase the body’s natural antioxidant production, enhance oxygen metabolism, and modulate the immune system.

Simply put, ozone is stimulating the body to balance and optimize important systems, such as the immune system, energy systems, blood circulation, and more.  

Proven Benefits

  • Block the virus’ ability to replicate by balancing the cellular redox state 2, 5, 16
  • Modulates the immune system 17, 18, 19
  • Improves oxygenation 20, 21, 22
  • Improves microcirculation
  • Corrects chronic oxidative stress

Theoretical benefits

  • When used with blood, deactivates the virus which creates a natural vaccine 23, 24, 25
  • Elimination of senescent cells.  Cells that are old are supposed to go through a self-destruction mechanism and eliminate themselves from the body.  However, senescent cells get old and never go through this process.  They build up and make the body less efficient

What the patient experiences

  • Increased energy and stamina - Due to improved oxygen efficiency and metabolism
  • Sense of wellbeing - Not entirely understood, but reported by top researchers
  • Decreased pain and discomfort - Reduces inflammation which may help to reduce pain.  Also calms down autoimmune discomfort by quieting down the immune system (immune modulator)
  • Improved clarity/decreased brain fog - Due to improved oxygen efficiency and metabolism

Ozone Therapy Uses

Ozone therapy diseases treated is a long list because of its fundamental improvement of systems in the body.

We have a complete list of diseases treated with ozone therapy here.

These are common reasons EBOO therapy is used:

  • Symptoms long after a viral infection
  • Lyme disease
  • Mold toxicity and fungal infections
  • Autoimmune conditions
  • Circulatory and cardiac conditions
  • Liver diseases and infections
  • Post-stroke
  • Cancer and patients undergoing chemotherapy
  • Athletic improvement and biohacking
  • Prevention of disease and anti-aging
  • Bacterial infections
  • Viral infections
  • Age related macular degeneration
  • Infectious disease
  • Ischemic diseases
  • Severe peripheral artery disease
  • Type 2 diabetes
  • Cardiac ischemia
  • Severe dyslipidemia
  • Necrotizing fasciitis
  • Chronic heart failure
  • Viral hepatitis type a, b and c
  • Acquired immune deficiency
  • Chronic inflammatory processes
  • Pre-treatment for patients who are planning to undergo antiviral therapy medication

And more

Is IV ozone safe?

We have put together a comprehensive safety review of ozone therapy that looks at all forms of literature on the subject.  You can read that here.

Ozone therapy safety is a concern to people because conventional medicine says, “ozone therapy is a scam”.  

However, it’s usually rooted in a lack of knowledge around the subject.  People don’t say this because they hold an in depth knowledge around the science of ozone therapy.  Usually they are making a sweeping judgment based on their paradigm.

In addition, it is well accepted by both ozone therapists and conventional practitioners that ozone cannot be breathed.  

Why can ozone be used in blood or other parts of the body if it cannot be breathed?

There is no antioxidant defense system in the lungs.  So when you breathe ozone gas it can cause irritation.  

Dr. Bocci established the scientific understanding of how ozone can be safely applied.  When ozone is infused into the blood, colon, or other areas used for ozone therapy, it interacts directly with the antioxidant system.  Your body is protected against the oxidative properties of ozone and cascades into the other benefits elicited by the treatment.  

Insert demonstration graphic (ozone cannot be breathed into lung.  No antioxidants.  It can be applied to other parts of the body where antioxidants are present.

A report was conducted by the World Federation of Ozone Therapy.  They evaluated over 300,000 ozone therapy treatments.  In the report, they found that ozone therapy had an adverse event rate of 6 out of 100,000 treatments.  All of which were minor. 

Ozone therapy has a higher safety record than the use of aspirin.

A good overview study was written on the safety of ozone therapy that displays this mechanism.

What are the side effects of ozone therapy?

The most notable negative side effect of ozone therapy is the herxheimer reaction.  A herxheimer reaction happens when the patient has an immune system burdened with viral, bacterial, or fungal infections.  Ozone therapy stimulates the immune system.  Then the immune system kills off lots of pathogens, creating a rapid release of endotoxins from the dead pathogens.  This can make people feel sick if it happens too quickly.

The largest IV ozone therapy risk is a herxheimer reaction.  This is not comfortable and can make the patient feel tired or sick for a few days.  

However, it’s avoidable by starting on a lower dose for immune burdened patients to lower ozone therapy risk.  The dose can be increased with each treatment until the standard dose is reached.

Are saline bags suitable for ozone IV treatment?

This is somewhat of a controversial topic, depending on who you ask.

Ozone is a strong oxidant.  Saline bags are made of plastics that break down in exposure to ozone.

Ozone reacts with blood almost instantaneously.  So if blood is in the bag, it should not interact with the bag.  

Most doctors are using saline bags in IV ozone therapy.  Clinically, it seems to work well without negative side effects.  But using glass or a bag specifically made for ozone is ideal.

Why do some doctors use UBI with IV ozone treatment?

Ozone ubi therapy is a combination of ozone IV and UV light therapy.  During the treatment, blood is dripped past a light chamber.  The idea of this ozone UBI treatment is to dismantle some of the bacteria and virus in the blood, then reinfuse it as an autovaccine.  It is also a form of photobiomodulation.

UBI ozone IV infusions are conveniently done together because it does not require much additional work on the doctor’s part.  

Light therapy IVs can be done as a standalone treatment or at the same time as ozone therapy.

UV light therapy IV with ozone appears to be safe and work synergistically.

How much does IV ozone therapy cost?

Ozone therapy cost is generally in the $150 - $300 per treatment.  Five to ten sessions are often prescribed.  But pricing can vary based on the clinic and location.  

IV ozone cost can also vary on the protocol, add-ons, packaging, and form of ozone therapy.

What IV ozone therapy equipment is needed?

The practitioner will generally spend around $10,000 for getting started with ozone therapy.  This includes the equipment and training.  You can find training on ozone ubi equipment here.

Is there ozone therapy training for doctors?

Yes, drsozone provides training on ozone therapy equipment, IV ozone therapy training, and ozone UBI training.  You can find it here.

Ozone therapy near me

To find ozone therapy clinics near you, email info@drsozone.com to find the closest clinic in your vicinity.

IV Ozone therapy major autohemotherapy protocol

How to do IV ozone therapy.


  1. Attach IV set to the 250cc saline bag and flush the IV line so no bubbles are present.  Then use the roller clamp to close the line.
  2. Draw up 500 units of heparin into a 1cc syringe then inject into empty 60cc syringe
  3. Have 3 pieces of tape ready for holding down the IV line on the patient

IV ozone protocol overview:

The goal is to draw 60ccs of blood from the patient, then push it into the bag.  Once the blood is in the bag, 60ccs of ozone gas will be added and then dripped back to the patient as quickly as desired.  

Full IV ozone therapy protocol

  1. Apply light tourniquet to patient to make veins easier to access
  2. Alcohol swab patient where needle will puncture
  3. Puncture desired vein with 21g butterfly needle or catheter
  4. Once blood is visible in the needle, use plastic forceps to clamp and prevent blood from leaking
  5. Tape down IV line on patient 
  6. Attach the heparinized 60cc syringe to luer lock on needle 
  7. Remove forceps then draw 60cc of blood into the syringe. detach the 60 cc syringe
  8. Attach the IV line with saline to the patients needle
  9. Attach an 18G needle to the heparinized blood-filled syringe and infuse the blood into the saline bag 
  10. With a 25-27G needle add ozone at 60cc of 50 mcg to the bag bubbling through the blood
  11. Gently mix blood and heparin/Saline mix (massage bag) 5 seconds
  12. Unclamp the IV line and set to 2 - 4 drops/sec.  It should take 25-25 minutes for drip time.
  13. When IV stops lift the line to allow more blood/saline mix flow into the patient
  14. Clamp off when blood/saline mix stops moving
  15. Remove needle and dispose of all blood products properly

How can I add laser light therapy to current UBI devices?  Maybe just change one of the bulbs?  Then make a special cuvette that works better with the laser light.  Or maybe there is a port that shoots laser light into the cuvette.

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