This HiDose Ozone Therapy Protocol is provided by the Society of Progressive Medical Education
- Draw up 40cc of saline use a 60cc syringe and 18g needle
- Prime the line: At the Hi-Dose cuvette Y port, infuse saline mix toward patient end, then clamp off
- With the cuvette held upright infuse the additional saline toward the spike port eliminating air from the cuvette
- Insert cuvette into UV machine and IV line into infusion pump (flow from patient to bag)
- Preload the syringe with 3,000-5,000 IU of heparin draw up 60cc of saline.
- Attach the small line to the syringe and Install the syringe into the syringe pump and set the pump to 150ml/hr speed and 50ml VTBI (volume to be infused.
- Set infusion pump rate at 1200 ml/h and transfer 330 ml (VTBI) of blood/saline from patient to bag.
- Should take 16 minutes
- Prepare patient, insert 19g - 21g needle into patient and attach to the cuvette luer end
- Turn on UBI device
- Turn on infusion pump
- Turn on syringe pump
- Once blood is done transferring, connect another syringe with 40cc of saline onto syringe line and purge lines both ways First to patient! so the HI Dose cuvette is diluted
- Clamp the line below the drip chamber prior to gas insertion
- Turn off the lights of the UBI unit. Turn them back on prior to reinfusion of the blood/mix
- Connect ozone generator to the bag via silicone tube and bubble at ¼ LPM at 70ug/ml for 4 minutes.
- The ozone generator is set at 70 Nmg/l, 15 l/hr, 4 minutes
- Disconnect silicone tube and replace cap on bag luer port with a non-vented luer lock cap
- Gently rock the bag to mix ozone with blood (ensure all ports are capped prior to mixing)
- Invert HI Dose bag – ensure that there is no air in the line
- Set the IV line in the pump so that it is directed back to the patient
- Set infusion pump rate at 1200 ml/h and 400ml and pump mixture back to patient. It will quit when line bubbles get to the pump.
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